Python virtualenvs what i do is that i edit the activate script and add tmux set-environment VIRTUAL_ENV $VIRTUAL_ENV after that, inside activate script there is deactivate function, i edit that function and add tmux set-environment -r VIRTUAL_ENV this way after i start venv all windows and panes will open with venv inside that session. If i deactivate new windows will open normal. ---- tmuxp.yaml in the root of the project. session_name: tmuxp start_directory: ./ # load session relative to config location (project root). before_script: ./.tmuxp-before-script.sh shell_command_before: - '[ -f .venv/bin/activate ] && source .venv/bin/activate && reset' windows: - window_name: tmuxp focus: True layout: main-horizontal options: main-pane-height: 35 panes: - focus: true - pane - make watch_test - window_name: docs layout: main-horizontal options: main-pane-height: 35 start_directory: docs/ panes: - focus: true - pane - make serve - make SPHINXBUILD='poetry run sphinx-build' watch ---------------- session_name: live-sdk start_directory: ../live-sdk before_script:./install.sh windows: - window_name: live-sdk focus: True layout: main-horizontal options: main-pain-height: 35 panes: - focus: true - pane - source sdk - load devuan devuan-desktop-metal chimaera - window_name: live-metal layout: main-horizontal options: main-pane-height: 35 start_directory: ./ panes: - focus: true - pane - ls .